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Project Discovery: Challenging & Interesting Samples

First post
Author
Galaxxis
Unicorn Rampage
#101 - 2016-03-16 20:21:37 UTC
http://i.imgur.com/ztbGdXM.png

Not really sure I agree with cytoplasm on this one.
HPA Illuminator
H P A
C C P Alliance
#102 - 2016-03-16 20:34:07 UTC
Galaxxis wrote:
http://i.imgur.com/ztbGdXM.png

Not really sure I agree with cytoplasm on this one.


Will check it!
PAPULA
Some Assembly Required.
#103 - 2016-03-16 20:48:18 UTC
Galaxxis
Unicorn Rampage
#104 - 2016-03-17 04:45:24 UTC  |  Edited by: Galaxxis
beakerax wrote:
I think this one was posted earlier, but here's the whole slide: http://i.imgur.com/clY9Uw5.jpg (100073159)

Metaphase stage of mitosis? fantastic image, whatever it is


Here's another one! http://i.imgur.com/MG3jQBa.jpg

There's one of those in the upper right corner. I had another one earlier with two of those side-by-side and what looked like centrosomes on each one. What are they?

Another one. They're everywhere! http://i.imgur.com/bOcz5zF.jpg

oh my god http://i.imgur.com/uAEFu67.jpg

Why are there so many of these all of a sudden? I don't think the earlier slides had so many.
HPA Illuminator
H P A
C C P Alliance
#105 - 2016-03-17 05:14:42 UTC
Galaxxis wrote:
beakerax wrote:
I think this one was posted earlier, but here's the whole slide: http://i.imgur.com/clY9Uw5.jpg (100073159)

Metaphase stage of mitosis? fantastic image, whatever it is


Here's another one! http://i.imgur.com/MG3jQBa.jpg

There's one of those in the upper right corner. I had another one earlier with two of those side-by-side and what looked like centrosomes on each one. What are they?

Another one. They're everywhere! http://i.imgur.com/bOcz5zF.jpg

oh my god http://i.imgur.com/uAEFu67.jpg

Why are there so many of these all of a sudden? I don't think the earlier slides had so many.


They are dividing cells (mitosis). What you see is DNA (blue) that has been duplicated and condensed and is now being pulled apart by the red.

The cells with cytokinetic bridges (nicely labeled :)) have just undergone mitosis and are about to/have just been disconnected there.

As for why more are appearing... Either we've in general become much better at looking for such cells over the years. I also think it matters how long the cells are allowed to grow before we stain them. 7 years ago, they only got to grow for 2-4h but now we keep them for 24h most of the time. In general cells duplicate around every 24-36h in vitro so it's far more likely to catch them in mitosis now. And they probably also feel better, getting a longer time to adhere to the slide,which increases chances of mitosis.

As for why you get so many in a row... Random!
Circumstantial Evidence
#106 - 2016-03-17 17:14:34 UTC
Sample 100000440 has been defined as 100% Vesicles, and I regret not making this choice, in addition to Rods and Rings. I picked R&R because I saw a few rod-like structures... am I completely wrong, or could this sample contain both R&R and Vesicles? Must complete ring structures be present, in samples with R&R? I've marked the BG views with arrows.

Area 1 BG -- RGB || Area 2 BG -- RGB


Sample 100000264 has been defined as 100% Cytoplasm, but I wonder what the structures that seem to outline some parts of the cells might be, marked with arrows in my Green-only screenshot. I thought they might represent a plasma membrane.

100000264 Green -- 100000264 RGB
Joia Crenca
Science and Trade Institute
Caldari State
#107 - 2016-03-17 23:45:05 UTC
I'd like to request this thread be as sticky as a Focal Adhesion
Joia Crenca
Science and Trade Institute
Caldari State
#108 - 2016-03-18 00:27:58 UTC
I have sample 100016981 that seems to me to be a 'nuclear bodies' one, but when I submit it, the community consensus is 100% vesicles? Odd. No screenshot, sorry.

Galaxxis
Unicorn Rampage
#109 - 2016-03-18 02:26:02 UTC  |  Edited by: Galaxxis
Guys, vesicles!

http://i.imgur.com/gZ7mtke.jpg
http://i.imgur.com/4y7CBQd.png

I think we need another thread for problem slides, since these aren't really interesting or challenging.
HPA Illuminator
H P A
C C P Alliance
#110 - 2016-03-18 08:23:35 UTC
Galaxxis wrote:
Guys, vesicles!

http://i.imgur.com/gZ7mtke.jpg
http://i.imgur.com/4y7CBQd.png

I think we need another thread for problem slides, since these aren't really interesting or challenging.


Perfect cell junctions! Don't think it's vesicles (was that a question?) as really random pattern all over cyto/nucleus, so doesn't seem specific.

If you start a new thread, please let me know so we can keep track of what you think is difficult & improve (and give relevant feedback).
Galaxxis
Unicorn Rampage
#111 - 2016-03-18 16:26:22 UTC
http://i.imgur.com/Lh4E8Pr.jpg

Suddenly I'm hungry for donuts.
Joia Crenca
Science and Trade Institute
Caldari State
#112 - 2016-03-18 18:07:52 UTC
People like to pick Cytoplasm for the strangest things.. Cytoplasm everywhere! (especially when one gets podded...)
Joe Thelere
The fun part
#113 - 2016-03-18 19:13:29 UTC
I had an image analysed by a professional today:
http://imgur.com/8cy3D7m
How much difference in intensity is needed to be correct with Cell-to-Cell-Variations? Or was the professional wrong?
Galaxxis
Unicorn Rampage
#114 - 2016-03-18 21:17:13 UTC
Joe Thelere wrote:
I had an image analysed by a professional today:
http://imgur.com/8cy3D7m
How much difference in intensity is needed to be correct with Cell-to-Cell-Variations? Or was the professional wrong?


Personally I would agree with you on that one. I've had several where it was either classified wrong or they missed something, so it happens from time to time. Presumably they'll go back and check if something is wrong with a control sample.
Selphentine
Pastafaris
#115 - 2016-03-18 21:51:22 UTC  |  Edited by: Selphentine
http://i.imgur.com/1jGFchx.jpg

Im by far noone that claims professionals are wrong, and i understand a lot of me seeing not the right things in PD, but im very, very sure that nucleoli shouldnt be wrong here.
Galaxxis
Unicorn Rampage
#116 - 2016-03-19 19:05:15 UTC  |  Edited by: Galaxxis
HPA Illuminator wrote:

If you start a new thread, please let me know so we can keep track of what you think is difficult & improve (and give relevant feedback).


Rather than start a new thread and have stuff spread out all over, we can probably just keep everything in this one.

This one is just completely broken. This isn't zoomed in on a section, it's just what the slide looks like.
http://i.imgur.com/YHtfNos.jpg

Here's another weird one, the red marker is messed up.
http://i.imgur.com/cuLvOB7.png
http://i.imgur.com/MBz7gpj.png
Galaxxis
Unicorn Rampage
#117 - 2016-03-20 00:24:01 UTC
HPA Illuminator wrote:
Galaxxis wrote:
I need to get an image hosting thing, I've had some really interesting ones. One looked like a nucleus popped and all the goo came out! It was glowing bright green and there was a black spot where the nucleus should have been.


Would love to see that one! :)


I finally got another one that looks a lot like it.
http://i.imgur.com/Qt6kI0b.png
http://i.imgur.com/SRMzdv0.png
Beta Maoye
#118 - 2016-03-20 03:32:12 UTC
Many different opinions on this one. http://uploadpie.com/RTkEo
Yllej Gniht
0rizen Irregulars
Sev3rance
#119 - 2016-03-24 14:54:56 UTC
Image no. 100000325

Classification Result says: "Nuclear Speckles"; it sure looks like "Nucleoplasm" to me.

What's the deal here? Is this faint variation enough to declassify it as "nucleoplasm" and flag it as "nuclear speckles"?

If so, some explaining text would be appreciated with those very hard to understand "teaching moments".

Thank you! :)
Memphis Baas
#120 - 2016-03-24 23:48:43 UTC
Why is the nucleus option wrong in this accuracy sample?
http://imgur.com/SjyQSxW

Please explain.