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Project Discovery: Challenging & Interesting Samples

First post
Author
March rabbit
Aliastra
Gallente Federation
#81 - 2016-03-14 19:21:36 UTC
I've heard that some images was analyzed by professionals? Shocked
screenshot

The Mittani: "the inappropriate drunked joke"

HPA Illuminator
H P A
C C P Alliance
#82 - 2016-03-14 22:05:02 UTC
Helios Anduath wrote:
Beta Maoye wrote:
I think this slide has two signals, microtubule organizing center and centrosome, not just microtubule organizing center.
RBG: http://uploadpie.com/onin4
Zoom 1: http://uploadpie.com/IHDCU
Zoom 2: http://uploadpie.com/wRlX5
Zoom 3: http://uploadpie.com/VlkIU


They don't really look like Centrosome because they are not two very well defined spots side by side. I would just have gone MTOC for this.


Based on zoom 2 I'd go for centrsosome and think the others cells were slightly out of focus.
HPA Illuminator
H P A
C C P Alliance
#83 - 2016-03-14 22:05:40 UTC
March rabbit wrote:
I've heard that some images was analyzed by professionals? Shocked
screenshot


Thanks for noticing, will double check it and correct if necessary.
beakerax
Pator Tech School
#84 - 2016-03-15 01:54:38 UTC
Galaxxis
Unicorn Rampage
#85 - 2016-03-15 02:48:45 UTC
beakerax wrote:


I had that one yesterday! I put cytoskeleton, although I'm really not sure if that's right.
Memphis Baas
#86 - 2016-03-15 03:06:14 UTC  |  Edited by: Memphis Baas
I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample.

A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that.
Galaxxis
Unicorn Rampage
#87 - 2016-03-15 04:16:38 UTC
Memphis Baas wrote:
I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample.

A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that.


I mean I agree that it kind of looks like that, and that was my thought as well, but then why is there still a nice round blue part visible? Shouldn't that be all scrunched up inside the green part?
Galaxxis
Unicorn Rampage
#88 - 2016-03-15 07:03:48 UTC
http://i.imgur.com/DGmPo87.png

I get these sometimes, but usually there's no dye anywhere except for a single bundle of microtubule ends somewhere. This one has green goo all over the place.
HPA Illuminator
H P A
C C P Alliance
#89 - 2016-03-15 07:07:20 UTC
Memphis Baas wrote:
I had that one just now; the splash of green overlaps the nucleus on every cell, so it can't really be anything outside the nucleus. I put Nuclear Membrane, Abnormal sample.

A Google images for "abnormal nuclear membrane stain" shows a couple examples where the membrane could fold in on itself or maybe shrink, to look slightly like that.


<3

Something happened to cells during prepp, so what you see is not something that is actually specific. I don't think it't the nuclear membrane but rather the plasma membrane that has shrunk (the nucleus still intact).

Have added to checkup list.
HPA Illuminator
H P A
C C P Alliance
#90 - 2016-03-15 07:08:39 UTC
Galaxxis wrote:
http://i.imgur.com/DGmPo87.png

I get these sometimes, but usually there's no dye anywhere except for a single bundle of microtubule ends somewhere. This one has green goo all over the place.


I'd say the green mostly is specific, and I'll be curious to see the results of it (I think some cells have been washed away during sample prep).
Galaxxis
Unicorn Rampage
#91 - 2016-03-16 00:22:57 UTC
beakerax
Pator Tech School
#92 - 2016-03-16 02:51:55 UTC  |  Edited by: beakerax
I think this one was posted earlier, but here's the whole slide: http://i.imgur.com/clY9Uw5.jpg (100073159)

Metaphase stage of mitosis? fantastic image, whatever it is
Memphis Baas
#93 - 2016-03-16 04:05:13 UTC


I would guess Plasma Membrane, Focal Adhesions, and Nuclear Speckles.
PAPULA
Some Assembly Required.
#94 - 2016-03-16 08:24:14 UTC  |  Edited by: PAPULA
Ok so, this game is totally wrong.

look at this sample:

http://www.netsky.org/eve/notcor2.png


As you can see it's wrong, but it's not.
I chose Nucleoplasm because it is Nucleoplasm, but game says no it is not.

Shocked

And the "correct answer" was: Nuclear speckles
Shocked
Helios Anduath
The Scope
Gallente Federation
#95 - 2016-03-16 11:02:37 UTC
PAPULA wrote:
Ok so, this game is totally wrong.

look at this sample:

http://www.netsky.org/eve/notcor2.png


As you can see it's wrong, but it's not.
I chose Nucleoplasm because it is Nucleoplasm, but game says no it is not.

Shocked

And the "correct answer" was: Nuclear speckles
Shocked


The game isn't wrong, it just has a mis-categorised control sample (though I would have gone nucleoplasm plus speckles because there looks to be speckling). HPA people have been quite good at seeing these posts and grabbing the mis-categorised controls.
Memphis Baas
#96 - 2016-03-16 15:34:28 UTC  |  Edited by: Memphis Baas
I think he's using the term "the game is wrong" because, although they are "quite good at seeing these posts and grabbing the mis-categorised controls" he still gets his accuracy penalized and losing the AK points for his armor or whatever he wants to get out of the game through no fault of his own.

EDIT: it's presented as an in-game activity, we get in-game rewards, people have a right to treat it as a game and, well, meta-game it.
Katsu Kho
Kho Incorporated
The Lone Space Wolves
#97 - 2016-03-16 20:12:03 UTC
I found this one to be a little weird...

Katsu Kho

Ambassador of the Jin-Mei Interstellar Space Bushidō council

Find me on YouTube - Latest video: Project Discovery Tutorial

Galaxxis
Unicorn Rampage
#98 - 2016-03-16 20:16:55 UTC  |  Edited by: Galaxxis
Katsu Kho wrote:
I found this one to be a little weird...


That's a great example of endoplasmic reticulum. See how it looks like a really thick spider web?
HPA Illuminator
H P A
C C P Alliance
#99 - 2016-03-16 20:18:03 UTC
PAPULA wrote:
Ok so, this game is totally wrong.

look at this sample:

http://www.netsky.org/eve/notcor2.png


As you can see it's wrong, but it's not.
I chose Nucleoplasm because it is Nucleoplasm, but game says no it is not.

Shocked

And the "correct answer" was: Nuclear speckles
Shocked


It's a known error. Really sorry for it, we're working on fixing the samples that incorrect and hope to have fixed it by this week. *crossing fingers* Will let ppl know when done.
HPA Illuminator
H P A
C C P Alliance
#100 - 2016-03-16 20:19:15 UTC
Galaxxis wrote:
Katsu Kho wrote:
I found this one to be a little weird...


That's a great example of endoplasmic reticulum. See how it looks like a really thick spider web?


What ^ said.

Also, there's a really nice staining of the nuclear membrane, which should also be seen for ER (they are connected). NB that only should be annotated ER.