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Project Discovery: Challenging & Interesting Samples

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HPA Illuminator
H P A
C C P Alliance
#161 - 2016-04-12 14:51:18 UTC
Raven Dallacort wrote:
I think there's an error on the classification for your control sample.

http://i.imgur.com/pWNR1bC.png

Thanks for your time.


Thanks for reporting, will look into it!
HPA Illuminator
H P A
C C P Alliance
#162 - 2016-04-12 14:53:54 UTC
Elyia Suze Nagala wrote:
I have no idea how some people are selecting a few of their answers in PD. It's annoying because I'll select features that are very clearly present, but no one else selected that feature type and I then get dinged a few pecent. I'm like WTH?

I still like the idea of PD but it's annoying my ratings are affected because others aren't thorough.


Consensus no longer affects accuracy rating in PD, it's only based on your coherence of control samples (of which a few, unfortunately, are incorrect atm).

If you come across control images that you disagree with, an imgur album collection is highly appreciated by us at HPA.

If you disagree with other peoples choices, the ingame chat is great for discussion about samples ("Project Discovery").

o/
Joia Crenca
Science and Trade Institute
Caldari State
#163 - 2016-04-14 20:38:01 UTC
Quote:
Consensus no longer affects accuracy rating in PD, it's only based on your coherence of control samples (of which a few, unfortunately, are incorrect atm).



Cool, I was posting on a different thread and didn't see any acknowledgement of the incorrect control samples there, so I'm glad to see it here.

Is it going to be possible to get a SOE Titan with cell samples as a skin with enough Analysis Kredits? Lol
Galaxxis
The Regency
The Monarchy
#164 - 2016-04-16 20:34:04 UTC
http://i.imgur.com/QKvGmwK.jpg

It looks like paintball splats.
Ristari
Federal Navy Academy
Gallente Federation
#165 - 2016-04-18 21:15:43 UTC
http://imgur.com/bLeQJPR

Cell division in metaphase/anaphase with the centrosomes lighting up. Thought this was a kind of cool find!

Would also like to take a moment to ask about the considerations you take when looking at nuclear staining, and this image happens to also contain what I've been thinking about for a while. In this image, you can clearly see that there's a difference in attenuation where the nucleoli are. It's very clear cut, and the attenuation difference matches the "holes" in the blue filter nucleus perfectly. The cytoplasm is however lighting up fairly well in this image too, and because of this, the nucleoli would appear to stain as well, since you have cytoplasm surrounding the nucleus. For this sample a lot of people went for nucleus. Nobody went for nucleoplasm. I'm thinking it's because the aforementioned "depth issues" causes confusion. Or am I getting this wrong?

Here's an image of the green filter:

http://imgur.com/JnfSGYL

While feeling pretty confident about the nucleoplasm staining, I was also thinking maybe there's no nuclear staining at all, like, maybe the difference in attenuation is due to the membrane lighting up? Or if I spot these nucleoli having a different attenuation compared to the surroundings, can I always be sure that the nucleoplasm is stained?

Sorry for the wall of text. Appreciate any input.
Ristari
Federal Navy Academy
Gallente Federation
#166 - 2016-04-18 21:29:14 UTC
Galaxxis wrote:
http://i.imgur.com/QKvGmwK.jpg

It looks like paintball splats.


Can sort of feel the panic in that image. The cells are ripped off the peaceful culture dish they grew up on and smeared on a dark and sterile glass slide. They're frantically reaching out for their loved ones in their moment of death. "Moooom!!! Hellllp!" "A431!! NOOOOOOO!!!!!" Sad
Raven Dallacort
Center for Advanced Studies
Gallente Federation
#167 - 2016-04-21 15:51:06 UTC
Could we take a look at this control sample as well please.

http://imgur.com/vqg6EmT

I believe both should be the correct answer.
Red Yxa
Freedom Buildiers Corp.
#168 - 2016-04-23 16:00:54 UTC
I think there should be some second answer
http://imgur.com/357Bguq
Galaxxis
The Regency
The Monarchy
#169 - 2016-04-23 16:04:46 UTC
Red Yxa wrote:
I think there should be some second answer
http://imgur.com/357Bguq


No.
Ristari
Federal Navy Academy
Gallente Federation
#170 - 2016-04-25 07:12:17 UTC
http://imgur.com/bvSxTO1

Cells exploding, burning, leaving trails of smoke... There's even a couple with bullet holes (0,1400µm Howitzer Artillery?). This is EVE! Just look at it. You can hear it too, can't you? "The fibroblast is primary! I repeat, the fibroblast is primary!" "Overheat ribosomes... vesicles! NOW!" "Plasma membrane down! PODOCYTE HIM!"

Ahem.
HPA Illuminator
H P A
C C P Alliance
#171 - 2016-04-29 13:32:13 UTC
Raven Dallacort wrote:
Could we take a look at this control sample as well please.

http://imgur.com/vqg6EmT

I believe both should be the correct answer.


There's an ongoing discussion in the group how to deal with nuclear speckles vs. nucleoplasm. We're leaning towards that when nuclear speckles are this dominant, one should not choose nucleus/nucleoplasm as well.
HPA Illuminator
H P A
C C P Alliance
#172 - 2016-04-29 13:35:49 UTC
Ristari wrote:
http://imgur.com/bLeQJPR

Cell division in metaphase/anaphase with the centrosomes lighting up. Thought this was a kind of cool find!

Would also like to take a moment to ask about the considerations you take when looking at nuclear staining, and this image happens to also contain what I've been thinking about for a while. In this image, you can clearly see that there's a difference in attenuation where the nucleoli are. It's very clear cut, and the attenuation difference matches the "holes" in the blue filter nucleus perfectly. The cytoplasm is however lighting up fairly well in this image too, and because of this, the nucleoli would appear to stain as well, since you have cytoplasm surrounding the nucleus. For this sample a lot of people went for nucleus. Nobody went for nucleoplasm. I'm thinking it's because the aforementioned "depth issues" causes confusion. Or am I getting this wrong?

Here's an image of the green filter:

http://imgur.com/JnfSGYL

While feeling pretty confident about the nucleoplasm staining, I was also thinking maybe there's no nuclear staining at all, like, maybe the difference in attenuation is due to the membrane lighting up? Or if I spot these nucleoli having a different attenuation compared to the surroundings, can I always be sure that the nucleoplasm is stained?

Sorry for the wall of text. Appreciate any input.


It's a bit late in the day, so I'm likely missing some aspects of your comment. But, if it was a nuclear staining, I'd absolutely say it was nucleoplasm and not nucleus. However, I'm not convinced it's really a nuclear staining, as it's just one cell with clear holes for nucleoli, which leads me to believe that it's likely a bleedthrough from the nuclear membrane (and some "background" staining).
Taira Arois
Arois Family Foundation
#173 - 2016-05-01 00:15:34 UTC
It seems that control sample 100455950 has been mislabled. The correct answer was Nuclear bodies (few) but there are more than 5 spots in every cell. This probably needs to be changed to Nuclear bodies (many).
Galaxxis
The Regency
The Monarchy
#174 - 2016-05-01 17:02:38 UTC
Taira Arois wrote:
It seems that control sample 100455950 has been mislabled. The correct answer was Nuclear bodies (few) but there are more than 5 spots in every cell. This probably needs to be changed to Nuclear bodies (many).


That's a problem with a lot of these. I've seen plenty of slides with one or two nuclear bodies in some and a bunch in others. Even if you select cell-to-cell variations you can only pick one of them, so which do you go with? Most of these have the community split down the middle as well. Is there a good reason to separate these? Couldn't this just be one button for "nuclear bodies"?
Aeligos
A.'.A.'.
#175 - 2016-05-02 02:10:49 UTC
Any chance we can get PD on our mobile devices?

Also, is there any way miners can contribute to PD? Ice, asteroids, can contain unique samples that can be uploaded to the PD database. The miner thus providing unique contributions to the cause. Would be kinda neat imo.

Smile

libera te tvtemet ex inferis

A.'.A.'.
HPA Illuminator
H P A
C C P Alliance
#176 - 2016-05-02 11:25:43 UTC
Taira Arois wrote:
It seems that control sample 100455950 has been mislabled. The correct answer was Nuclear bodies (few) but there are more than 5 spots in every cell. This probably needs to be changed to Nuclear bodies (many).


Please report in to this reddit thread, where we try collect everything:

https://www.reddit.com/r/Eve/comments/4gtoyg/project_discovery_collecting_incorrect_control/
HPA Illuminator
H P A
C C P Alliance
#177 - 2016-05-02 11:30:29 UTC
Galaxxis wrote:
Taira Arois wrote:
It seems that control sample 100455950 has been mislabled. The correct answer was Nuclear bodies (few) but there are more than 5 spots in every cell. This probably needs to be changed to Nuclear bodies (many).


That's a problem with a lot of these. I've seen plenty of slides with one or two nuclear bodies in some and a bunch in others. Even if you select cell-to-cell variations you can only pick one of them, so which do you go with? Most of these have the community split down the middle as well. Is there a good reason to separate these? Couldn't this just be one button for "nuclear bodies"?


Nuclear bodies is one category where you're really helping us, as we've only said "nuclear bodies" internally, and it's a quite recent category, so we've probably missed out on a lot of samples! Getting out information on many/few is super useful for us! So if there are samples where ppl vote 50/50 it will be interesting for us to look at those (nuclear bodies might eg. correspond to DNA damage site, binding to certain DNA regions etc, so it's not surprising that they may differ between cells).

As for which category to go for... I'd go for the dominant one (most cells). If it's really 50/50, I'd chose many, as the NB are really small, so the reason for just seeing a few in some cells might be due to focus being slightly off (but if you go for few, it's not a problem!).
Kolmogorow
Freedom Resources
#178 - 2016-05-04 20:44:14 UTC
This image had quite a low consensus and I was very unsure how to classify it as well. First thought was: Mitochondria, but it doesn't look very "thread-like", at least I had seen images that looked quite more clearly like Mitochondria. For ER which was another possible choice it didn't look enough to me like a "spider web". Decided just for cytoplasm at the end but I'm not convinced at all. Hmmmm, what is it actually?
HPA Illuminator
H P A
C C P Alliance
#179 - 2016-05-05 18:41:13 UTC
Kolmogorow wrote:
This image had quite a low consensus and I was very unsure how to classify it as well. First thought was: Mitochondria, but it doesn't look very "thread-like", at least I had seen images that looked quite more clearly like Mitochondria. For ER which was another possible choice it didn't look enough to me like a "spider web". Decided just for cytoplasm at the end but I'm not convinced at all. Hmmmm, what is it actually?



It's kinda hard to tell with all colors (do you have green only?) but Im thinking mitochondria.
Kolmogorow
Freedom Resources
#180 - 2016-05-05 20:20:41 UTC
HPA Illuminator wrote:
Kolmogorow wrote:
This image had quite a low consensus and I was very unsure how to classify it as well. First thought was: Mitochondria, but it doesn't look very "thread-like", at least I had seen images that looked quite more clearly like Mitochondria. For ER which was another possible choice it didn't look enough to me like a "spider web". Decided just for cytoplasm at the end but I'm not convinced at all. Hmmmm, what is it actually?



It's kinda hard to tell with all colors (do you have green only?) but Im thinking mitochondria.


Don't have the green only image anymore, but on a second look I believe as well that Mitochondria would have been the better choice. Thanks for the feedback!