EVE General Discussion

it's hard to say without the color filters

keep in mind that it can be cytoplasm and nucleoplasm/nucleoplasm in the same picture.

it leans towards cytoplasm, if the red area is well stained with the green marker, maybe, but not necessarily concentraded around the blue area, but can also be equally spread throughout the whole cell. add vesicles if there are bright green dots.

it leans towards nucleoplasm/nucleus if only the blue part is stained.

in any case, you need a visible difference between the two areas to mark either. if there is NO difference, pick weak or undefined.

if there is only very weak staining in the red area, don't pick cytoplasm.

i would say that's plasma membrane in your picture since the green marker spills outside of the red one. it could be more than that, but that's not really visible without the filters.

you can have both at the same time. you just need a visible difference between the two areas to make a distinction.

i'm trying to find an example for you right now, give me a few minutes :)

Hey man first off thank you for taking the effort to explain this ;)

I would fully agree to you about the Nucleoplasm, your description fits the way I look at it. I also agree that the plasmatic membrane is incorrect. But I disagree to the 90% cytoplasm result.

To quote myself, cytoplasm: 'all the red might be green but the blue is blue without a spec of green in it.'
Your imagine is showing green all over the cells (including the blue area's). shouldn't that mean it is defiantly NOT cytopalsm

@Nick

once again. you can have multiple things going on at the same time. the green staining in the blue area comes from nucleoplasm, whereas the green staining in the red area comes from cytoplasm

this is an example of pure cytoplasm (+ vesicles)

https://gyazo.com/597b71ec73291b5b1f0fce9b64ec7cd4

the faint green spots in this zoom from the middle section

https://gyazo.com/d0bf894db0a7f870772ca1caf7917045

is just from more cytoplasm that is on top or below the nucleus. (you are looking at a 2D image of a 3D structure)

Was this a question? I would say it's either cell junctions of plasma membrane. I would lean towards plasma membrane because in the upper left and lower right parts you can see some staining outside the cells. Possibly both CJ+PM is correct, that's also an option :)

There are some cases like that, and if the nucleoli is prominent, i'd go for nucleus + nucleoli.

In this case, I don't think the overlap is good, it looks like nucleoplasm+speckles to me (just like you've chosen).

lol pacman hungry

But I bet it tells you two of the options are wrong, and 60% choose cytoplasm, because if project discovery isn't outright glitching it's most people selecting cytoplasm.

http://i.imgur.com/kVBI0tR.jpg

Look at that crap, it's clearly a negative. But half of the awnsers are cytoplasm. I'm trying to learn this game, but it seems a lot of people aren't even insterested. I wouldn't be surprised if people were running macros.

with that mindset things would start making more sense. If I'm allowed to hide the green with the blue then it could indeed be both at the same time. I still find it somewhat strange but seeing as most people do it and that most results of the test server where correct....I must have been wrong.

Thanks to you (and the others) for all the help :)

They don't really look like Centrosome because they are not two very well defined spots side by side. I would just have gone MTOC for this.