EVE General Discussion

Post all the really hard, or really strange samples you've seen so far... For Science!
Try to include the ID number in the lower right corner. It might be useful for ccp/mmos to fix things.

Not sure whats going on in this one. Its like a couple of them exploded or something. This is your cells on drugs?
http://i.imgur.com/dNKrZQ1.png

This one i got semi-right.. apparently it had 3 different classifications i needed to select, i only got the most obvious one.
http://i.imgur.com/M9yq6FU.png

Heres one i was almost certain i was right, but apparently not. The staining was clearly outside of the red marker, uniform though the whole thing with no difference in the blue marker area, and with pointy protrusions extending out, like the Plasma Membrane, but it turned out to be Cytoplasm? Cytoplasm description says it should be uniform inside the cell, except in the blue marker, and i dont see any of these protrusions in any of the cytoplasm examples.
http://i.imgur.com/tpulJhH.png


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Other good posts in this thread worth checking out:

Detailed information about some of the images posted in the thread by someone who knows something
https://forums.eveonline.com/default.aspx?g=posts&m=6386145#post6386145

An example of cell-to-cell Variation and description of whats being stained:
https://forums.eveonline.com/default.aspx?g=posts&m=6386238#post6386238

heres one that nobody seems sure of, look at the spread of those numbers!
http://i.imgur.com/F2QMP1K.png

and heres one that doesnt seem to fit any description. Its probably not a Microtubule Organizing Center, since none of the red converges on it at all, and they are fairly solid dots, not diffuse. It might be a Centrosome but again, none of the red converges on it, and its kinda randomly placed in the red. It cant be an Aggresome, since its often not next to the nucleus, and is not diffuse.
I marked it as 'abnormal sample' and chose Aggresome anyway since its the closest match i could find.
http://i.imgur.com/R56dMoD.png

"you are helping to do science" simple explanation or "you are helping to identify what structures are present in pretty pictures of really small things" simple explanation?

Basically, you are looking at pictures of stained human cells to identify patterns of protein distribution in them. The aim is to map all of the proteins in the human body to help understand health and disease.

The ones that are Red Cross/Green Outline on the results like that (or titled "Foreign cell sample") are ones that have been classified by researchers at HPA - player's haven't come to that consensus.

Ones that have been classified by players show you the percentages in the results and are the unknown samples.

Am I missing something here?

https://i.gyazo.com/bc19dab4632c6ab7a48d22522b6b8381.png

The first few days, the results are going to be all over the map because we have a little learning of our own to do.

I tryed to do it but i have no idea what i am doing.
I read all the toturial and instructions but i always miss the correct answer.

Remember these are 3D images, so there can be green under the blue, which isn't 'In' the blue but part of the cell literally underneath.

What a fun thread!

Some feedback on your difficult images from a researchers pov:

#1: First image shows fibrillar centers. You can tell because the green staining will overlap with the holes in the blue one. It's FC rather than nucleoli, as there are clusters of spots in each nucleoli (FC is a specific pattern of nucleolar staining, with a specific biological function).

The cells that look like they've exploded are actually cells that are undergoing mitosis = one cell becoming two. So what you see is the DNA (blue) all densed up, so the microtubules (red) can help separate the DNA equally into the two daughter cells. Not so common to see this in images, and soooo pretty imo :)

Second image: Difficult one, agree that the nucleoli is the most prominent staining (it's just really important to toggle all colors on/off).

#3: Hehe you're really finding difficult ones! I would say it's nucleoli, but I'm not sure whether it's "just" nucleoli, or whether it's fibrillar center. I don't think it's nuclear bodies because the green seem to overlap with the holes in the blue. It's not speckles because there are too few and not evenly spread out.

Second image: Tricky one! The spots are really dense and oversaturated. It might be some sort of vesicle or cytoplasmic body, but it could also be an artifact. Typical image where there's no obvious right or wrong.

#6: Totally agree with you on both! There might be a cytoplasmic staining together with the plasma membrane (those two can be really difficult to tell apart). Are you getting errors for choosing PM?

#9: Can't say for sure with both the blue and green turned on, but if looks like nucleus rather than nucleoplasm to me.

#10: You're right, it's mitochondria. I can sort of understand why they could be mistaken for vesicles though... but that thread like pattern in a part of the cell really gives it away.

#12: Ooooh, those were some ugly cells. I can tell from just looking at the morphology and size that those belong to one of the first few 1000 cell preps we did (to give some perspective, we've done >150.000 now & we've improved a lot). It's a really difficult one to distinguish, but I think it might be intermediate filaments. It could also be "just" cytoplasm. My reason for thinking of intermediate filaments is that the green is a bit more dense next to the nucleus, and it's kinda like a cotton pad that you've ripped apart (at work we usually go like "uuuh so it's not golgi, cytoplasm or mitochondria... it must be int fil..." - we also think it's one of the more difficult ones!).

Nice one! I wouldn't say it's abnormal. It's a cell to cell variation pattern of plasma membrane. Possibly some cytoplasm too (but likely not) - if the green staining is perfectly uniform and flat all over the cell when you toggle off red and blue I would say it's only PM.